We believe that the glass with the film will protect the fungi the most, followed in efficiency by the glass because the glass helps reflect the UV rays away from direct contact with the fungi. The least protective will be the straight UV rays onto the fungi, because there is no protection from the rays. We hypothesize that the longer the fungi is exposed (no matter what filter is used) the more damage the UV lamp will have on its growth. By the time that we got around to doing the experiment we only ended up testing timed exposure, rather than the films.
Journal Article
Solar UV-B Radiation Inhibits the Growth of Antarctic Terrestrial Fungi. (n.d.). National Center for Biotechnology Information. Retrieved January 9, 2012, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150076/
Discipline. (n.d.). JSTOR: An Error Occurred Setting Your User Cookie. JSTOR: An Error Occurred Setting Your User Cookie. Retrieved January 9, 2012, from http://www.jstor.org/stable/3869507
Lab Procedure (Source?)
Didn't do: Different shields
1. Obtain a petri dish and put fungi in it
2. Put a glass shield over the petri dish with the fungi in it and put a UV light over the glass
3. Leave the light on for 30 minutes and check back to see how much fungi is left
4. Repeat two more times, once with nothing covering the fungi, and another time with a glass shield and a film covering the fungi
5. Record how many fungi are left
Different times
1. Obtain a petri dish and put fingi in it
2. Put a UV light over the fungi for 10 minutes
3. Record how many fungi are left
4. Repeat two more times, once for 20 minutes, and another time for 30 minutes
5. Record how many fungi are left
A dark room is also needed to perform the experiment accurately, as the UV needs to be the only light effecting the fungi.
Safety Information: Chemicals/Reaction
The only dangerous aspect is the UV light to the eyes, so protective eye wear must be warn while operating the UV lamp at all times. Proper lab precautions will be taken. This means rubber gloves and glasses when working with materials.
Other Information
Abstract:
THE IMPACT OF UV RAYS ON FUNGI.Brigid Connelly and Emily Nasseff. The purpose of this lab was to discover and observe the impact that UV rays have on fungi. By testing the effect of UV rays on fungi growth, this experiment observed the damage of UV rays to other cell growth. The first step was to apply fungi to homemade potato agar located in Petri dishes. The same amount of fungi sample was spread to 28 Petri, carefully as so not to further contaminate the agar. The dishes were then individually placed in a UV cabinet and exposed to the rays for a certain period of time. The first two trials were done for 5, 20, and 60 seconds while the third, fourth, and fifth trials each had a set of 5 minute, 10 minute, and 15minute Petri dishes. Finally, the sixth and seventh trial each had an exposure time of 30 minutes. The results showed no connection between the periods of exposure to the growth of the fungi. Every dish exposed grew the same amount (in the same amount of time) as its constant. After 3-4 days, picture evidence proves that UV rays had no impact on this specific type of fungus. Keywords: Fungi, Aspergillius Niger, UV rays, Potato Agar
Journal Articles:
Caldwell, Bjorn, Bornman, Flint, Kulandaivelu, Teramura, Tevini. (1998). Effects of Increased Solar Ultraviolet Radiation on Terrestrial Ecosystems. Journal of Photochemistry and Photobiology. Retrieved from:http://www.sciencedirect.com/science/article/pii/S1011134498001845.
Discipline. (n.d.). JSTOR: An Error Occurred Setting Your User Cookie. JSTOR: An Error Occurred Setting Your User Cookie. Retrieved January 9, 2012, from http://www.jstor.org/stable/3869507
Different shields
1. Obtain a petri dish and put fungi in it
2. Put a glass shield over the petri dish with the fungi in it and put a UV light over the glass
3. Leave the light on for 30 minutes and check back to see how much fungi is left
4. Repeat two more times, once with nothing covering the fungi, and another time with a glass shield and a film covering the fungi
5. Record how many fungi are left
Different times
1. Obtain a petri dish and put fingi in it
2. Put a UV light over the fungi for 10 minutes
3. Record how many fungi are left
4. Repeat two more times, once for 20 minutes, and another time for 30 minutes
5. Record how many fungi are left
http://mypages.iit.edu/~smile/bi8802.html
The purpose of this lab was to discover and observe the impact that UV rays have on fungi. By testing the effect of UV rays on fungi growth, this experiment observed the damage of UV rays to other cell growth. The first step was to apply fungi to homemade potato agar located in Petri dishes. The same amount of fungi sample was spread to 28 Petri, carefully as so not to further contaminate the agar. The dishes were then individually placed in a UV cabinet and exposed to the rays for a certain period of time. The first two trials were done for 5, 20, and 60 seconds while the third, fourth, and fifth trials each had a set of 5 minute, 10 minute, and 15minute Petri dishes. Finally, the sixth and seventh trial each had an exposure time of 30 minutes. The results showed no connection between the periods of exposure to the growth of the fungi. Every dish exposed grew the same amount (in the same amount of time) as its constant. After 3-4 days, picture evidence proves that UV rays had no impact on this specific type of fungus.
Keywords: Fungi, Aspergillius Niger, UV rays, Potato Agar
Caldwell, Bjorn, Bornman, Flint, Kulandaivelu, Teramura, Tevini. (1998). Effects of Increased Solar Ultraviolet Radiation on Terrestrial Ecosystems. Journal of Photochemistry and Photobiology. Retrieved from:http://www.sciencedirect.com/science/article/pii/S1011134498001845.
Cockell, C., Knowland, J. (1999).Ultraviolet Radiation Screening Compounds. Biological Reviews. Retrieved From:
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-185X.1999.tb00189.x/abstract.
Netting, Ruth. (2007). Ultraviolet Light From Our Sun. NASA. Retrieved from: http://missionscience.nasa.gov/ems/10_ultravioletwaves.html.
Stapleton, A. (1992). Ultraviolet Radiation and Plants: Burning Questions. American Society of Plant Physiologists. Retrieved from:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC160223/pdf/041353.pdf.
UV Radiation and Cancer. American Cancer Society. (2007). Retrieved from: http://www.cancer.org/acs/groups/content/@nho/documents/document/uvradiationandcancerpdf.pdf.